Authors: Ortwin Naujok Joanna Kaldrack Terbish Taivankhuu Anne Jörns Sigurd Lenzen
Publish Date: 2010/04/22
Volume: 6, Issue: 3, Pages: 450-461
Abstract
Pluripotent cell lines such as embryonic stem cells are an attractive source for a potential cell replacement therapy However transplantation of differentiated cells harbors the risk of teratoma formation presenting a serious health risk To overcome this obstacle a negative selection system was established that permits selective removal of undifferentiated cells during in vitro differentiation Use of the HSV1 thymidine kinase and eGFP under the control of the Oct4 promoter allowed the destruction of undifferentiated ES cells by ganciclovir treatment differentiated cells were unharmed Clonal ES cells remained pluripotent and showed positive staining for a wide range of embryonic markers Thus treatment with ganciclovir during in vitro differentiation effectively removed the population of undifferentiated cells and provided a pure population of completely differentiated cells This approach may pave the way for a safe application of ES cells in regenerative medicine in the future
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