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Title of Journal: Stem Cell Rev and Rep

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Abbravation: Stem Cell Reviews and Reports

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Humana Press Inc

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1558-6804

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Calcium Homeostasis in Human Induced Pluripotent S

Authors: YeeKi Lee KwongMan Ng WingHon Lai YauChi Chan YeeMan Lau Qizhou Lian HungFat Tse ChungWah Siu
Publish Date: 2011/05/26
Volume: 7, Issue: 4, Pages: 976-986
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Abstract

Cardiomyocytes generated from human induced pluripotent stem cells hiPSCs are suggested as the most promising candidate to replenish cardiomyocyte loss in regenerative medicine Little is known about their calcium homeostasis the key process underlying excitationcontraction couplingWe differentiated cardiomyocytes from hiPSCs IMR90 and KS1 and hESCs H7 and HES3 with established protocols Beating outgrowths from embryoid bodies were typically observed 2 weeks after induction Cells in these outgrowths were stained positively for tropomyosin and sarcomeric alphaactinin Reversetranscription polymerase chain reaction studies demonstrated the expressions of cardiacspecific markers in both hiPSC and hESCderived cardiomyocytes Calcium handling properties of 20dayold hiPSC and hESCderived cardiomyocytes were investigated using fluorescence confocal microscopy Compared with hESCderived cardiomyocytes spontaneous calcium transients from both lines of hiPSCderived cardiomyocytes were of significantly smaller amplitude and with slower maximal upstroke velocity Better caffeineinduced calcium handling kinetics in hESCCMs indicates a higher sacroplasmic recticulum calcium store Furthermore in contrast with hESCderived cardiomyocytes ryanodine did not reduce the amplitudes maximal upstroke and decay velocity of calcium transients of hiPSCderived cardiomyocytes In addition spatial inhomogeneity in temporal properties of calcium transients across the width of cardiomyocytes was more pronounced in hiPSCderived cardiomyocytes than their hESC counterpart as revealed linescan calcium imaging Expressions of the key calciumhandling proteins including ryanodine recptor2 RyR2 sacroplasmic recticulum calciumATPase SERCA junction Jun and triadin TRDN were significantly lower in hiPSC than in hESCs

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Other Papers In This Journal:
  1. Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells
  2. The Stem Cell Research Environment: A Patchwork of Patchworks
  3. Selective Removal of Undifferentiated Embryonic Stem Cells from Differentiation Cultures Through HSV1 Thymidine Kinase and Ganciclovir Treatment
  4. New Approaches in the Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Hepatocytes
  5. Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells
  6. Claudins and Cancer Stem Cells
  7. Present Status and Perspectives of Stem Cell-Based Therapies for Gastrointestinal Diseases
  8. Emerging Ethical, Legal and Social Issues Associated with Stem Cell Research & and the Current Role of the Moral Status of the Embryo
  9. Physiological Electrical Signals Promote Chain Migration of Neuroblasts by Up-Regulating P2Y1 Purinergic Receptors and Enhancing Cell Adhesion
  10. The Effects of Nuclear Reprogramming on Mitochondrial DNA Replication
  11. Wnt/Catenin Signaling in Adult Stem Cell Physiology and Disease
  12. Endothelial Cells in Co-culture Enhance Embryonic Stem Cell Differentiation to Pancreatic Progenitors and Insulin-Producing Cells through BMP Signaling
  13. Generation of Cancerous Neural Stem Cells Forming Glial Tumor by Oncogenic Stimulation
  14. Cytoskeletal Disassembly and Cell Rounding Promotes Adipogenesis from ES Cells
  15. Fibrin-Embedded Adipose Derived Stem Cells Enhance Skin Flap Survival
  16. Molecular Advances in Reporter Genes: The Need to Witness the Function of Stem Cells in Failing Heart in Vivo
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