Journal Title
Title of Journal: Plant Foods Hum Nutr
|
Abbravation: Plant Foods for Human Nutrition
|
|
|
|
|
|
|
|
Authors: Syed Sultan Beevi Lakshmi Narasu Mangamoori Murugan Subathra Jyotheeswara Reddy Edula
Publish Date: 2010/07/23
Volume: 65, Issue: 3, Pages: 200-209
Abstract
Raphanus sativus a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties It has been used in indigenous system of medicine for the treatment of various human ailments in India This present study evaluated the chemopreventive efficacy of different parts of R sativus such as root stem and leaves extracted with solvents of varying polarity and investigated the molecular mechanism leading to growth arrest and apoptotic cell death in human cancer cell lines Of the different parts significant growth inhibitory effect was observed with hexane extract of R sativus root Analysis of hexane extract by GCMS revealed the presence of several isothiocyanates ITCs such as 4methylthio3butenyl isothiocyanate MTBITC 4methylthio3butyl isothiocyanate erucin 4methylpentyl isothiocyanate 4pentenyl isothiocyanate and sulforaphene R sativus root extract induced cell death both in p53 proficient and p53 deficient cell lines through induction of apoptotic signaling pathway regardless of the p53 status of cells The molecular mechanisms underlying R sativusinduced apoptosis may involve interactions among Bcl2 family genes as evidenced by upregulation of proapoptotic genes and downregulation of antiapoptotic genes along with activation of Caspase3 Our findings present the first evidence that hexane extract of R sativus root exerts potential chemopreventive efficacy and induces apoptosis in cancer cell lines through modulation of genes involved in apoptotic signaling pathwayThis study was supported by a funding under the Technology Education Quality Improvement Program TEQIP by World Bank to Centre for Biotechnology Institute of Science and Technology Jawaharlal Nehru Technological University Hyderabad India The first author acknowledges the financial support from CSIR in the form of a Senior Research Fellowship
Keywords:
.
|
Other Papers In This Journal:
|