Multipulsed high hydrostatic pressure treatment f

Authors: Mutlu PilavtepeÇelik Sencer Buzrul Hami Alpas Alain Largeteau Gérard Demazeau

Publish Date: 2010/08/10

Volume: 6, Issue: 3, Pages: 343-348

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Abstract

Escherichia coli cells in peptone water were pressurized at 300 MPa at ambient temperature with no holding time pulse series and with a total holding duration of 300 s for single 300 s × 1 pulse and multipulsed 150 s × 2 pulses 100 s × 3 pulses 75 s × 4 pulses 60 s × 5 pulses 50 s × 6 pulses and 30 s × 10 pulses high hydrostatic pressure HHP treatments Multipulsed HHP treatment with no holding time indicated that as the pulse number increased the number of inactivated and injured cells also increased Holding time had significant effect on the inactivation of E coli There was low inactivation difference between single and multipulsed HHP treatments with holding time Escherichia coli cells showed at least 16 log10 more reduction on selective medium than the nonselective medium indicating that more than 95  of the survivors severely injured for both single and multipulsed treatments with holding time Although the inactivation difference was low between single and multipulsed HHP treatments storage at 4 °C revealed that there was less recovery from injury for multipulsed HHP treatment

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