Species confirmation of fungal isolates by molecul

Authors: Rupesh Thakur Sardul S Sandhu

Publish Date: 2010/10/30

Volume: 50, Issue: 3, Pages: 280-291

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Abstract

Traditional taxonomy of hyphomycetes has been based on conidial morphology and development In order to confirm species level for the detection and identification of the entomopathogenic fungus we analysed the speciesspecific fingerprints to investigate molecular characteristics within isolates of six species and to resolve morphologically atypical isolates The extent of fingerprint profile observed by RAPD was sufficient to confirm the species level of all the isolates The genetic similarity among morphologically identified isolates of each species was considerably higher allowing us to conclude that all the isolates are of same species These results establish a molecular framework for further taxonomic phylogenetic and comparative biological investigations

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