Novel Class 1 Integrons in Multidrug Resistant Is

Authors: Guangming Sun Maoli Yi Chen Shao Jin Ma Qiang Zhang Shihe Shao

Publish Date: 2013/12/04

Volume: 54, Issue: 2, Pages: 227-231

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Abstract

Integrons are mobile genetic elements able to capture express and excise resistance genes playing an important role in the spread of bacterial resistance The present study was to investigate the occurrence and diversity of integrons in 120 clinical multidrug resistant Gramnegative isolates from eastern China Screening of integrons was performed by PCR and gene cassettes were further characterized by PCR–RFLP and sequencing Class 1 integrons were detected in 708  of isolates and no class 2 and class 3 integrons were detected in any isolates A total of 19 resistant gene cassettes were identified four representative of novel gene cassettes an aacA3 variant aacA3c an aacA4 variant aacA4′17 a bla OXA variant bla OXA251 and a catB8 gene cassette interrupted by an insertion sequence IS10 catB8IS10 In addition 14 cassette arrays were detected including three novel integrons gcuD1aacA4′17gcu38BcatB8IS10 In712 aacA3caadA13bla OXA251 In713 and dfrA1gcu37aadA5 In714 The presence of novel integron structures in clinical isolates suggests hospital environments may favor the formation of novel combination of gene cassettes Moreover the high prevalence of integrons in multidrug resistant isolates highlights the urgent need to employ effective means to avoid dissemination of drugresistant bacteriaThis work was partly supported by the PhD Programs foundation of the Ministry of Education of China No 20113227120008 the Natural Science Foundation of Jiangsu Province No BK2012289 the foundation of Society Development of Zhenjiang City No 2010041

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