Journal Title
Title of Journal: Indian J Microbiol
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Abbravation: Indian Journal of Microbiology
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Publisher
Springer-Verlag
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Authors: Hua Wang Shiteng Huang Jianzhong Zhao Jun Han Xianwei Guan Shihe Shao
Publish Date: 2012/12/15
Volume: 53, Issue: 1, Pages: 36-40
Abstract
Helicobacter pylori H pylori is a highly successful humanspecific gastric pathogen infecting over half the world’s population Virulent H pylori isolates harbour the cytotoxinassociated genes pathogenicity island cagPAI the majority of which have no known function In this study we used cell infection assay and reverse transcriptase PCR identified that CagL recombinant protein one of the cagPAI proteins induced GES1 cells to express cytokine IL8 Then we performed western blot and translocation assay Our result showed CagL polyclonal antibody counteracted translocation of CagA This will provide a foundation for the further studies on its biological functionWe thank Prof Seungchul Baik Gyeongsang National University College of Medicine Republic of Korea for kindly providing us the suicide plasmid pBlueKM40 and technical assistant for mutant construct of H pylori This work was supported by grants from the National Natural Science Funds 81271795 and Research Foundation for Advanced Talents in Jiangsu University 12JDG064
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