Authors: V S Shubina V P Lavrovskaya E N Bezgina L L Pavlik D A Moshkov
Publish Date: 2010/12/11
Volume: 41, Issue: 1, Pages: 1-5
Abstract
BHK21 cells were incubated in medium containing dopamine DA and catecholamine contents were then measured using the Falck cytochemical method As compared with controls significant increases were seen in the fluorescence of cells and these were proportional to the concentration and duration of exposure to DA and more marked in cells in suspension than in attached cells Parallel electron microscopic studies showed that the increased fluorescence intensity of the cytoplasm correlated with the presence of dense networks of fibrils which were identified on the basis of morphological characteristics as microfilaments consisting of Factin Prior blockade of dopaminergic receptors with haloperidol did not alter the subsequent effects of DA on fluorescence intensity or cell ultrastructure These data suggest that in conditions of chronic exposure DA can penetrate into the cytoplasm inducing actin polymerization and becoming bound to the newly formed actin cytoskeleton Structurally this can be apparent as hypertrophy of the cytoskeleton and its derivatives with significant influences on the overall structure of the cell
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