Authors: Hengky Kurniawan JinnTsyy Lai MengJiy Wang
Publish Date: 2012/09/23
Volume: 19, Issue: 6, Pages: 1975-1988
Abstract
Bacterial cellulose BC membranes were modified with oxygen O2 nitrogen N2 and tetrafluoromethane CF4 plasmas in order to enhance cell affinity The surface properties of the pristine and plasmatreated BCs were analyzed through water contact angles electron spectroscopy for chemical analysis ESCA and scanning electron microscopy O2 and N2 plasmas changed the surface of BCs to more hydrophilic while CF4 plasma altered BCs to be very hydrophobic ESCA analyses indicated that O2 N2 and CF4 plasmas incorporated the functionalities of carbon–oxygen amides and amino and carbonfluoride on BCs respectively The effects of the plasma treatments on the adhesion of L929 fibroblast and Chinese hamster ovary cell lines showed that the cell adhesion and proliferation of both cells was significantly improved on BCCF4 in contrast with that on the pristine BC BCO2 and BCN2 revealing a functionalityspecific effect resulted from different plasmas Moreover protein adsorption tests indicated that a higher quantity of proteins in cell culture medium was adsorbed on the CF4 plasmatreated BCs which presumably played the role of enhancing the subsequent cell growth This work highlights the great potential of plasma treatments on the improvement of biocompatibility and surface property of BCs for biomedical applicationsThe authors would like to demonstrate great appreciation to the financial supports from National Science Council of Taiwan NSC 992621M011003 the Food Industry Research and Development Institute Taiwan FIRDI and National Taiwan University of Science and Technology
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