Effects of carbon black to inflammation and oxidat

Authors: KyungTaek Rim SooJin Kim JeongHee Han MinGu Kang JongKyu Kim JeongSun Yang

Publish Date: 2012/01/04

Volume: 7, Issue: 4, Pages: 415-423

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Abstract

Carbon black is classified as carcinogen group 2B by International Agency for Research on Cancer IARC But it uncertained the effects of ultrafine carbon black particles on oxidative damage or inflammation So we were focused to evaluate the oxidative damage or inflammation with ultrafine carbon black particles at gene expression level by using mouse macrophage cell line and the coeffects with solvent coating to it It was evaluated the changes of gene expression with real time RTPCR and oxidative DNA damage with Fragment Length Analysis with Restriction Enzyme FLARE assay in mouse macrophage RAW2647 cell line Two kinds of carbon black induced the gene expression of cytokines related to acute inflammation and with 01 methylcyclohexane coating were regulated conversely each other The oxidative DNA damage with smaller size carbon black was increased than bigger one the range with 500–30 nm The 01 methylcyclohexane increased the damage by binding with each carbon black the dose range with 100 ng/mL100 μg/mL In this study we got the conclusion that the genotoxicity of carbon blacks are elevated with its size get smaller and their surface area wider and with methylcyclohexane coating It could cause DNA damage by promoting oxidative stress and inflammatory responses

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