Cytotoxicity of venom from crownofthorns starfis

Authors: ChiChiu Lee WannSheng Tsai HernyiJustin Hsieh DengFwu Hwang

Publish Date: 2013/07/18

Volume: 9, Issue: 2, Pages: 177-184

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Abstract

The cytotoxic effects of the crownofthorns starfish Acanthaster planci spine venom ASV in five cell lines including human neuroblastoma SHSY5Y human hepatocellular carcinoma HepG2 human melanoma A375S2 human skin fibroblast CCD966SK and mouse macrophagelike cell RAW 2647 were assayed The results indicated that ASV showed cytotoxic effects depending on dose in these five cell lines Specifically ASV significantly inhibited the proliferation of human melanoma cell line A375S2 at 10 μg/mL indicating A planci spine venom could be utilized as potential chemotherapeutic agent in the treatment of cancer The cytotoxicity of ASV to human melanoma cell line A375S2 was relatively well retained at temperature less than 40°C and sharply lost at temperature more than 80°C The cytotoxicity of ASV also sharply lost at extreme pH environments pH 2 and 12 The cytotoxicity of ASV was attenuated when treated with Cu2+ and antioxidant Nacetylcysteine After SDSPAGE analysis ASV showed the major protein components ranging from 10 kDa to 37 kDa

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