A fabricated microfluidic paperbased analytical d

Authors: JaYoung Kim MinKyeong Yeo

Publish Date: 2016/04/01

Volume: 12, Issue: 1, Pages: 101-109

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Abstract

paperbased analytical device μPAD for in situ rapid colorimetric detection of microorganisms in environmental water samples We fabricated the μPAD with the wax printing method at curing conditions of 100°C for 7 seconds For quick bacterial detection we modified the catalase test with Fenton’s reaction as a colorimetric biochemical reaction This modified catalase test provided a color indicator according to the concentration of Escherichia coli E coli XL1 Blue strain All of the image data from the detection chamber of the μPAD was analyzed at the precision of 300 pixels for each color space component ie hue saturation and value in HSV color In addition environmental samples were tested with our μPAD and the data were fitted in the range of colorimetric reference chart for E coli sample testingTwenty microliters of 50 hydrogen peroxide solution was used for a singlecolor detection test Before adding the E coli sample on the chip the detection chambers were loaded twice with 1 μL of ferrous sulfate 03 g/mL 097 M and dried The mean values for all of the samples n=10 were rounded to the nearest integer The P values P001 P005 for all three of the color space components of HSV were considered to be statistically significant

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