Authors: Lili Zhang Lanwei Zhang Huaxi Yi Ming Du Yingchun Zhang Xue Han Zhen Feng Jingyan Li Yuehua Jiao Yanhe Zhang Chunfeng Guo
Publish Date: 2012/03/09
Volume: 29, Issue: 7, Pages: 913-917
Abstract
Stressmediated bioprocess is a strategy designed to enhance biological target productivity In this study an attempt was made to enhance transglutaminase TGase production by Streptomyces mobaraensis by using different stress conditions including heat shock alcohols and salt stress Results showed that the effects of stress on TGase production depended on the type applied For heat shock TGase production 132 U/ml was recorded maximum in the culture treated at 48 h post inoculation in water bath at 60 °C for 1 min For alcohols treatment the maximum activity of TGase 177 and 175 U/ml was obtained when 3 methanol was added into the medium at 0 or 24 h of fermentation However a 35fold increased production of TGase 38 U/ml was observed in the medium supplemented with 02 mol/l MgCl2 compared with the basic medium at the beginning of fermentation In conclusion TGase production from S mobaraensis was improved by heat shock methanol and salt stress treatments MgCl2 stress was the most effective
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