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Title of Journal: Korean J Chem Eng

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Abbravation: Korean Journal of Chemical Engineering

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Springer US

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DOI

10.1002/ibd.21836

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1975-7220

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Highly efficient molecular delivery into Emphasis

Authors: Kiyeon Jeon Arumuganainar Suresh YeuChun Kim
Publish Date: 2013/07/23
Volume: 30, Issue: 8, Pages: 1626-1630
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Abstract

Electroporation is a highly efficient delivery method for transformation in various cell types however in microalgae there is lack of optimized electroporation parameters due to cell wall protoplast preparation and viability Therefore we optimized electroporation conditions for transforming microalgae using Chlamydomonas reinhardtii strains of wild type and mutant cell wall deficient To investigate the effects of molecule size calcein 623 Da and fluorescein isothiocyanatedextran FITCdextran 40 kDa were used and various electroporation parameters were applied such as different voltage and pulse length and molecule uptake pattern and cell viability were observed Cell wall is insignificant in case of small sized molecule uptake as noticed by 125 kV/cm and 30 ms for both strains whereas for larger molecules by 15 and 2 kV/cm and 30 ms for mutant and wild type respectively In terms of viability there was no significant difference in both the strains on applied electroporation parameters The controlled parameters corresponding to 15 to 20 kV/cm and 20 to 30 ms could be used to deliver macromolecules DNA proteins into cells effectively

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