Journal Title
Title of Journal: Cell Tissue Bank
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Abbravation: Cell and Tissue Banking
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Publisher
Springer Netherlands
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Authors: Amin Bigham Sadegh Ehsan Basiri Ahmad Oryan Pezhman Mirshokraei
Publish Date: 2013/06/21
Volume: 15, Issue: 1, Pages: 127-137
Abstract
Adipose derived adult stem cells ASCs are multipotent cells that are able to differentiate into osteoblasts in presence of certain factors The histological characteristics of periosteum makes it a specific tissue with a unique capacity to be engineered Higher flexibility of the greater omentum is useful for reconstructive surgery These criteria make it suitable for tissue engineering The present study was designed to evaluate bone tissue engineering with periosteal free graft concurrent with ASCs and pedicle omentum in dog model Twelve young female indigenous dogs were used in this experiment In omental group n = 4 end of omentum was wrapped by periosteum of the radial bone in abdomen of each dog In omentalautogenously ASCs group n = 4 1 ml of ASCs was injected into the wrapped omentum with periosteum while in omentalallogenously ASCs group n = 4 1 ml of allogenous ASCs was injected Lateral view radiographs were taken from the abdominal cavity postoperatively at the 2nd 4th 6th and 8th weeks postsurgery Eight weeks after operation the dogs were reanesthetized and the wrapped omenum by periosteum in all groups was found and removed for histopathological evaluation Our results showed that omentum–periosteum omentalperiosteumautogenous ASCs and omentalperiosteumallogenous ASCs groups demonstrated bone tissue formation in the abdominal cavity in dog model The radiological macroscopical and histological findings of the present study by the end of 8 weeks postsurgery indicate bone tissue engineering in all three groups in an equal level The present study has shown that the wrapped omentum with periosteum concurrent with ASCs autogenous or allogenous ASCs lead to a favorable bone tissue formation We suggested that it may be useful when pedicle graft omentum used concurrent with periosteum in the bone defect reconstruction and this phenomenon should be studied in future
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