Authors: Fereydoon Aflaki Vanik Ghoulipour Nader Saemian Shahab Sheibani Maryam Salahinejad
Publish Date: 2014/10/14
Volume: 9, Issue: 1, Pages: 86-94
Abstract
Biogenic amines forms in food by microbial enzymatic decarboxylation of amino acids and excessive consumption of these amines can be of health concern Many techniques have been developed for the extraction of biogenic amines in food including acidic or organic extraction as well as solid phase extraction In this study we described an alternative method based on a stirrer bead milling for extraction of biogenic amines from fish muscles The rate and stirring time were optimized with an experimental design optimization procedure The developed method was compared with the traditional homogenization extraction method After a derivatization step with benzoyl chloride samples were analyzed for amines quantitative determination with RPHPLC system The analysis method showed good linearity correlation coefficients of 09989 and good recoveries 9423 The repeatability and reproducibility of the method in terms of the relative standard deviation ranged from 16 to 26 and 33 to 49 respectively Moreover the detection limits of biogenic amines were calculated between 005 and 009 mg kg−1 in fish samples The proposed method has been applied to the analysis of different fish species of the Persian Gulf Low biogenic amine levels were found in most fish samples Total biogenic amine contents of selected samples were ≤683 mg kg−1
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