Emphasis Type="SmallCaps"l/Emphasiscarnitine

Authors: R Soumya H Carl R Vani

Publish Date: 2015/07/11

Volume: 32, Issue: 3, Pages: 328-334

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Abstract

Erythrocytes undergo various changes during storage storage lesion that in turn reduces their functioning and survival Oxidative stress plays a major role in the storage lesion and antioxidants can be used to combat this stress This study elucidates the effects of lcarnitine LC on erythrocytes of stored blood Blood was obtained from male Wistar rats and stored 4 °C for 20 days in CPDA1 citrate phosphate dextrose adenine solution Samples were divided into–i controls ii LC 10 lcarnitine at a concentration of 10 mM iii LC 30 lcarnitine at a concentration of 30 mM and iv LC 60 lcarnitine at a concentration of 60 mM Every fifth day the biomarkers haemoglobin hemolysis antioxidant enzymes lipid peroxidation and protein oxidation products were analysed in erythrocytes Hemoglobin and protein sulfhydryls were insignificant during storage indicative of the maintenance of hemoglobin and sulfhydryls in all groups Superoxide dismutase and malondialdehyde levels increased initially and decreased towards the end of storage The levels of catalase and glutathione peroxidase were lower in experimentals than controls during storage lcarnitine assisted the enzymes by scavenging the reactive oxygen species produced Hemolysis increased in all groups with storage elucidating that lcarnitine could not completely protect lipids and proteins from oxidative stress Hence this study opens up new avenues of using lcarnitine as a component of storage solutions with combinations of antioxidants in order to maintain efficacy of erythrocytes

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