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Title of Journal: Trees

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Abbravation: Trees

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Springer Berlin Heidelberg

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DOI

10.1007/bf02539054

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1432-2285

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Reference genes selection for quantitative gene ex

Authors: Hu Chen Zhangqi Yang Ying Hu Jianhui Tan Jie Jia Hulan Xu Xinhua Chen
Publish Date: 2015/12/01
Volume: 30, Issue: 3, Pages: 685-696
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Abstract

An important prerequisite for obtaining accurate gene expression results using quantitative realtime PCR is the selection of a reference gene or a group of genes having a highly stable level of expression Pinus massoniana L PM is the predominant fastgrowing timber forest tree species in southern China In this study of PM we evaluated various tissues flowers in different developmental phases leaves from a cultivar with insect resistance and leaves from plants under several types of abiotic stresses Comprehensive Analysis was performed using BestKeeper Normfinder geNorm and RefFinder software to select the most stable reference gene or gene group from among 25 candidate genes in these samples The results showed that different experimental conditions require the use of different reference genes ACT1 could be used as a reference gene for all samples in this study UBI4 was the best gene for various tissues and zinc stress CYP was the most stable gene for leaves from insectresistant materials and Pb stress Fbox and UBI11 were the best reference genes for salt stress Fbox + RRP8 ARF + TUBA and EF1B + IDH were the best reference groups for drought stress low temperature stress and flowers in different developmental phases respectively This study presents a reliable selection of reference genes for Masson pine and the conclusions are meaningful for improving the accuracy of expression analyses in future molecular biology studiesThis project is partially supported by The special fund for Bagui scholar 2011A015 and Natural Science Foundation of Guangxi Zhuang Autonomous Region Grant No 2014jjBA30102 and Basic scientific research fund of Guangxi Forestry Research InstituteChina Grant No Linke 201410 and Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation 13A0302 13B0301 and Key Scientific and Technological project of Guangxi Zhuang Autonomous Region Grant No 11230044A

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