Anatomical distribution of primary amine oxidase a

Authors: Christian Carpéné Francisco Les Mounia Hasnaoui Simon Biron Picard Marceau Denis Richard Jean Galitzky Denis R Joanisse Pascale Mauriège

Publish Date: 2016/10/21

Volume: 73, Issue: 3, Pages: 475-486

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Abstract

Semicarbazidesensitive amine oxidase SSAO identical to primary amine oxidase or vascular adhesion protein1 is a membrane enzyme that generates hydrogen peroxide SSAO is highly expressed at the adipocyte surface and its plasma levels increase with type 2 diabetes Since visceral adipose tissue AT is more tightly associated with obesity complications than subcutaneous SC abdominal fat we compared SSAO activity in plasma and 4 distinct AT locations in 48 severely obese women body mass index BMI averaging 54 ± 11 kg/m2 with or without a dysmetabolic profile Higher glucose and triacylglycerol levels vs lower highdensity lipoprotein HDLcholesterol characterized dysmetabolic women DYS n = 25 from nondysmetabolic NDYS n = 23 age and weightmatched subjects SC mesenteric ME omental OM and round ligament RL fat locations were collected during bariatric surgery SSAO capacity to oxidize up to 1 mM benzylamine was determined in AT and plasma with radiometric and fluorimetric methods Plasma SSAO was higher in the DYS group SSAO activity was higher in fat than in plasma when expressed as radiolabeled benzaldehyde per milligram of protein In ATs from DYS women protein content was 10  higher and basal hydrogen peroxide release lower than in NDYS subjects except for RL location The SSAO affinity towards benzylamine did not exhibit regional variation and was not altered by a dysmetabolic profile K m averaging 184 ± 7 μM n = 183 Although radiometric and fluorimetric methods gave different estimates of oxidase activity both indicated that AT SSAO activity did not vary according to anatomical location and/or metabolic status in severely obese womenThe severely obese cohort was supported over the years by the Laval University MerckFrosst/CIHR Research Chair in Obesity We would like to express our gratitude to Drs Vicky Drapeau and Fanny Therrien for their helpful assistance in AT and plasma bank management The cooperation of subjects who participated to this study is also greatly appreciated Part of this work was supported by the operating grant OHP no 67388 obtained from the Canadian Institutes of Health Research by Pascale Mauriège European Erasmus funds supported F Les The authors express gratitude to Anne Bouloumié I2MC Toulouse and Victor Lopez San Jorge University for facilitating the scientific exchanges

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