A highaffinity folate binding protein in normal h

Authors: Jan Holm Steen Ingemann Hansen J⊘rgen Lyngbye

Publish Date: 1985/08/01

Volume: 5, Issue: 8, Pages: 683-688

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Abstract

Highaffinity binding of 3Hfolate to supernatant from homogenized human leukocytes containing large amounts of binding protein displayed apparent positive cooperativity The DEAESepharose® CL6B chromatographic profile of the supernatant at pH 63 contained a major peak of folate binding Mr approx 25 000 in the front effluent and a smaller more acidic peak Mr approx 25 000 that emerged after a rise in NaCl from 30 mmol/l to 1 mol/l Triton X100 solubilized ceil sediment from the leukocyte homogenate contained some highaffinity folate binding activity Mr approx 25 000 typically 5–10 of the total binding activity

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