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Abbravation: Bioscience Reports

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Portland Press Limited

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10.1002/mrm.24609

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1573-4935

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Protein Detection Methods in Proteomics Research

Authors: Reiner Westermeier Rita Marouga
Publish Date: 2005/02/04
Volume: 25, Issue: 1-2, Pages: 19-32
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Abstract

In proteomics research chemical as well as physical methods are used to detect proteins subsequently to their separation Physical methods are mostly applied after chromatography They are either based on spectroscopy like light absorption at certain wavelengths or mass determination of peptides and their fragments with mass spectrometry Chemical methods are used after twodimensional electrophoresis and employ staining with organic dyes metal chelates fluorescent dyes complexing with silver or prelabeling with fluorophores In some cases autoradiography is still used Since all of these techniques are very different in terms of sensitivity their usefulness for quantitative determinations varies significantly This review will describe the various protein detection methods applied to electrophoresis gelsAbbreviatons 2D twodimensional CCD charge coupled device DIGE difference gel electrophoresis EDTA Ethylenediaminetetraacetic acid ICAT isotope coded affinity tag MALDI ToF matrix assisted laser desorption ionization time of flight NMR nuclear magnetic resonance PTM posttranslational modification PAS periodic acid/Schiffs reagent PVDF Polyvinylidenefluoride SDS sodium dodecyl sulphate Tris Trishydroxymethylaminoethane


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  1. In vitro and in vivo effect of chloropromazine, imipramine and lithium chloride on monoamine oxidase activity in rat brain mitochondria
  2. Monoclonal antibodies to human plasma Protein X alias complement S-protein
  3. Vaccination today and tomorrow
  4. Mitochondrial DNA Medicine
  5. Heat shock proteins induce pores in membranes
  6. A high-affinity folate binding protein in normal human leukocytes: Ligand binding characteristics, ionic charge and molecular size
  7. Late signals are required for the stimulation of DNA synthesis in rat mammary fibroblasts by growth factors
  8. Gene conversion in avian mesotocin and vasotocin genes: A recurrent mechanism linking two neurohypophysial precursor lineages?
  9. Mitochondrial Optic Neuropathies: How Two Genomes may Kill the Same Cell Type?
  10. Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA)
  11. World Health and the Oxford International Biomedical Centre
  12. Interaction of quercetin with DNA
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  14. Proteoglycan-type I collagen fibril interactions in bone and non-calcifying connective tissues
  15. Activation of mammalian skeletal-muscle carbonic anhydrase III by arginine modification
  16. Mitochondria and Neurodegeneration
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