Protein Detection Methods in Proteomics Research

Authors: Reiner Westermeier Rita Marouga

Publish Date: 2005/02/04

Volume: 25, Issue: 1-2, Pages: 19-32

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Abstract

In proteomics research chemical as well as physical methods are used to detect proteins subsequently to their separation Physical methods are mostly applied after chromatography They are either based on spectroscopy like light absorption at certain wavelengths or mass determination of peptides and their fragments with mass spectrometry Chemical methods are used after twodimensional electrophoresis and employ staining with organic dyes metal chelates fluorescent dyes complexing with silver or prelabeling with fluorophores In some cases autoradiography is still used Since all of these techniques are very different in terms of sensitivity their usefulness for quantitative determinations varies significantly This review will describe the various protein detection methods applied to electrophoresis gelsAbbreviatons 2D twodimensional CCD charge coupled device DIGE difference gel electrophoresis EDTA Ethylenediaminetetraacetic acid ICAT isotope coded affinity tag MALDI ToF matrix assisted laser desorption ionization time of flight NMR nuclear magnetic resonance PTM posttranslational modification PAS periodic acid/Schiffs reagent PVDF Polyvinylidenefluoride SDS sodium dodecyl sulphate Tris Trishydroxymethylaminoethane

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