Authors: Retno Yusiasih Tsuyoshi Yoshimura Toshiaki Umezawa Yuji Imamura
Publish Date: 2003/08
Volume: 49, Issue: 4, Pages: 377-
Abstract
A method for screening wood extractives was developed using cellulose thinlayer chromatography plate CellTLC separation and was directly applied to bioassays Wood meal ≪60 mesh from nangka Artocarpus heterophyllus Lamk heartwood was extracted with hot methanol The crude extract was separated using a CellTLC plate 50 × 50 mm Two broad bands with R f values of 046 and 096 were found and the bands showed completely different effects against the pest termite Coptotermes formosanus Shiraki and the decay fungus Fomitopsis palustris The band with R f 046 was preferentially consumed by workers of C formosanus and it did not show any growth inhibition against F palustris when the CellTLC plate was directly exposed to the organism In contrast the band with R f 096 appeared to repel strongly the feeding by C formosanus and inhibited the growth of F palustris It was concluded that the CellTLC system was applicable for screening wood extracts consisting of many compounds
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