Authors: Pauline Leverrier Johannes P C Vissers Annette Rouault Patrick Boyaval Gwénaël Jan
Publish Date: 2004/01/17
Volume: 181, Issue: 3, Pages: 215-230
Abstract
Microorganisms used in food technology and probiotics are exposed to technological and digestive stresses respectively Traditionally used as Swisstype cheese starters propionibacteria also constitute promising human probiotics Stress tolerance and crossprotection in Propionibacterium freudenreichii were thus examined after exposure to heat acid or bile salts stresses Adapted cells demonstrated acquired homologous tolerance Crossprotection between bile salts and heat adaptation was demonstrated By contrast bile salts pretreatment sensitized cells to acid challenge and vice versa Surprisingly heat and acid responses did not present significant crossprotection in P freudenreichii During adaptations important changes in cellular protein synthesis were observed using twodimensional electrophoresis While global protein synthesis decreased several proteins were overexpressed during stress adaptations Thirtyfour proteins were induced by acid pretreatment 34 by bile salts pretreatment and 26 by heat pretreatment Six proteins are common to all stresses and represent general stressresponse components Among these polypeptides general stress chaperones and proteins involved in energetic metabolism oxidative stress response or SOS response were identified These results bring new insight into the tolerance of P freudenreichii to heat acid and bile salts and should be taken into consideration in the development of probiotic preparationsStanda Industrie is greatly acknowledged for financial support and for constant interest and enthusiasm in this work P Leverrier is the recipient of a grant from the Institut National de la Recherche Agronomique and from Standa Industrie John Hannon is acknowledged for English correction
Keywords: