Authors: Tamara F Kondrat’eva Vasily N Danilevich Svetlana N Ageeva Grigory I Karavaiko
Publish Date: 2005/07/19
Volume: 183, Issue: 6, Pages: 401-410
Abstract
IS elements were identified in the genomes of five Acidithiobacillus ferrooxidans strains isolated from various media IST2 elements were revealed in all the strains grown in a medium with ferrous iron ISAfe1 elements were detected in four strains TFBk TFL2 TFV1 and TFO Three strains TFV1 TFNd and TFO were found to contain IS elements ~600 bp long These were named preliminary as ISAfe600 Partial sequencing of the 5′ and 3′terminal nucleotide stretches of an ISAfe1 element in TFBk and TFL2 strains and complete sequencing of the ISAfe1 element in the TFBk strain has revealed nucleotide substitutions as compared to the prototype ie the ISAfe1 element of an ATCC 19859 strain Partial sequencing of the 5′ and 3′terminal nucleotide stretches of the IST2 elements in TFO TFBk and TFL2 strains has shown numerous nucleotide substitutions when compared to the IST2 element of an ATCC 19859 strain Complete sequencing of the IST2 element in the TFBk strain has revealed the divergence between the IST2 elements in the TFBk strain and the prototype was 212 Southern hybridization of EcoRI fragments of the chromosomal DNA from five A ferrooxidans strains grown in a medium with ferrous iron using an internal region of ISAfe1 a fulllength ISAfe1 or a fulllength IST2 as probes has shown them to differ in the number of copies of IS elements and their localization on the chromosomes Adaptation to elemental sulfur in A ferrooxidans strains caused changes in the number intensity and localization of hybridization bands The authors discuss the role of IS elements in the adaptation of A ferrooxidans to the new energy substrateThe nucleotide sequence data reported in this paper were deposited in GenBank under accession numbers AY823401 the ISAfe1 from A ferrooxidans TFBk AY825254 the IST2 from TFBk DQ002894 the 5′terminal nucleotide sequence of ISAfe1 from TFL2 DQ002895 the 3′terminal nucleotide sequence of ISAfe1 from TFL2 DQ005952 the 5′terminal nucleotide sequence of IST2 from TFV1 DQ005953 the 3′terminal nucleotide sequence of IST2 from TFV1
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