Authors: Anna Casabianca Chiara Orlandi Federica Barbieri Luigia Sabatini Andrea Di Cesare Davide Sisti Sonia Pasquaroli Mauro Magnani Barbara Citterio
Publish Date: 2014/12/23
Volume: 197, Issue: 3, Pages: 431-438
Abstract
Aeromonas hydrophila is an aquatic bacterium responsible for several human illnesses The aim of this work was to investigate the survival ability and virulence expression of two strains from different sources fish strain 87 and surface water strain LS maintained in a seawater microcosm The strains were analyzed for the total and viable bacterial counts adhesion ability to Hep2 cells and aerA gene expression by qPCR throughout the experiment 35 days Both strains reached a putative VBNC state and lost adhesive properties but exhibited a different behavior in the expression of aerA This could be due to the different origin of the two strains the former adapted to a habitat rich of nutrient and the latter already used to survive in a more hostile environment Moreover our results indicate that the quantitative determination of aerA mRNA can be a useful indicator of virulence expression under stress conditions
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