Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: J Biol Inorg Chem

Search In Journal Title:

Abbravation: JBIC Journal of Biological Inorganic Chemistry

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1016/0030-4220(50)90076-4

Search In DOI:

ISSN

1432-1327

Search In ISSN:
Search In Title Of Papers:

Effect of complex formation between ZnSuperscript

Authors: Suman Das Pukhrambam Grihanjali Devi Sudipta Pal Dipak Dasgupta
Publish Date: 2004/11/18
Volume: 10, Issue: 1, Pages: 25-32
PDF Link

Abstract

Mithramycin MTR a member of the aureolic group of anticancer antibiotics is a drug that supposedly acts via the inhibition of transcription It binds to bivalent cations such as Mg2+ and Zn2+ In this paper we report the association of MTR with Zn2+ a biologically important bivalent cation whose coordination property leads to its important role as a cofactor in different enzymes and nucleosomal DNAbinding proteins First we have characterized the complex formation between MTR and Zn2+ using spectroscopic methods In the second part we have examined the effect of the association of Zn2+ with MTR on the enzymatic activity of a typical zincIIdependent alcohol dehydrogenase ADH from yeast Our data show that MTR forms a single complex with Zn2+ in a mole ratio of 21 in terms of MTRZn2+ We also observe a negative effect for the preincubation of ADH with MTR upon the enzymatic activity These results indicate that MTR induced structural changes in the enzyme as a sequel to its complex formation with Zn2+ present in the enzyme thereby leading to a loss of enzymatic activity

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. The Fox1 ferroxidase of Chlamydomonas reinhardtii : a new multicopper oxidase structural paradigm
  2. Impaired hydrolysis of cisplatin derivatives to aquated species prevents energy-dependent uptake in GLC4 cells resistant to cisplatin
  3. Folding of the prion peptide GGGTHSQW around the copper(II) ion: identifying the oxygen donor ligand at neutral pH and probing the proximity of the tryptophan residue to the copper ion
  4. Rates of intercalator-driven platination of DNA determined by a restriction enzyme cleavage inhibition assay
  5. Rates of intercalator-driven platination of DNA determined by a restriction enzyme cleavage inhibition assay
  6. New vanadium-based magnetic resonance imaging probes: clinical potential for early detection of cancer
  7. DNA targeting and cleavage by an engineered metalloprotein dimer
  8. Cobalt hexaamine mediated electrocatalytic voltammetry of dimethyl sulfoxide reductase: driving force effects on catalysis
  9. Ciliate metallothioneins: unique microbial eukaryotic heavy-metal-binder molecules
  10. Structural and thermodynamical properties of Cu II amyloid- β 16/28 complexes associated with Alzheimer’s disease
  11. Sensitivity of the kinase activity of human vaccinia-related kinase proteins to toxic metals
  12. The role of axial ligands for the structure and function of chlorophylls
  13. Synthesis and structural characterization of soluble neuromelanin analogs provides important clues to its biosynthesis
  14. Synthesis and crystal structures of novel copper(II) complexes with glycine and substituted phenanthrolines: reactivity towards DNA/BSA and in vitro cytotoxic and antimicrobial evaluation
  15. Enantiopure copper(II) complex of natural product rosin derivative: DNA binding, DNA cleavage and cytotoxicity
  16. Human copper transporter Ctr1 is functional in Drosophila , revealing a high degree of conservation between mammals and insects
  17. A spectroscopic and voltammetric study of the pH-dependent Cu(II) coordination to the peptide GGGTH: relevance to the fifth Cu(II) site in the prion protein
  18. Kinetic and structural studies on roles of the serine ligand and a strictly conserved tyrosine residue in nitrile hydratase
  19. A realistic in silico model for structure/function studies of molybdenum–copper CO dehydrogenase
  20. Associating a negatively charged GdDOTA-derivative to the Pittsburgh compound B for targeting Aβ amyloid aggregates
  21. A systematic investigation of multiheme c -type cytochromes in prokaryotes
  22. Protein stability and mutations in the axial methionine loop of a minimal cytochrome c
Search Result: