Authors: ShangYin Vanson Liu ChangFeng Dai TungYung Fan HonTsen Yu
Publish Date: 2005/03/11
Volume: 7, Issue: 1, Pages: 26-32
Abstract
We attempted to isolate microsatellites from a Symbiodiniumfree gorgonian octocoral Junceella juncea using two methods partial genomic library screening and enrichment Among the 3856 clones screened by the partial library method 10 possibly positive signals were found and 3 of them could be used to design primers and amplified consistently In contrast only one locus isolated by the enrichment method gave reliable amplification and was useful The results indicate that microsatellites are rare in Junceella juncea as reported for other cnidarians Overall we obtained 4 polymorphic loci to test the feasibility in investigating clonal structure of J juncea A total of 40 multilocus genotypes were found among 152 colonies and the number of genotypes clones identified at 7 reefs ranged from 2 to 16 The results of a nonmetric multidimensional scaling analysis indicated the recruitment of J juncea populations mainly comes from selfretention These novel microsatellite loci will provide a useful tool to study clonal structure and population genetics for J juncea in the future
Keywords: