Protection of Shrimp against White Spot Syndrome V

Authors: Fei Zhu Xiaobo Zhang

Publish Date: 2011/05/18

Volume: 14, Issue: 1, Pages: 63-68

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Abstract

White spot syndrome virus WSSV is a major shrimp viral pathogen responsible for large economic losses to shrimp aquaculture all over the world The RNAi mediated by siRNA contributes a new strategy to control this viral disease However the efficient approach to deliver the siRNA into shrimp remains to be addressed In this investigation an antiviral vp28siRNA was encapsulated in β13dglucan and then the β13dglucanencapsulated vp28siRNA particles GeRPs were delivered into Marsupenaeus japonicus shrimp The results showed that the vp28siRNA in GeRPs could be released in hemocytes of shrimp It was found that the GeRPs containing the vp28siRNA inhibited the replication of WSSV in vivo which presented a better antiviral activity than the nonencapsulated vp28siRNA Further evidence indicated that the mortality of WSSVinfected shrimp was significantly delayed by the GeRPs containing vp28siRNA Therefore our study presented that the glucanencapsulated siRNA might represent a novel potential therapeutic or preventive approach to control the shrimp diseaseThis work was financially supported by National Natural Science Foundation of China 30830084 31001127 HiTech Research and Development Program of China 863 program of China 2010AA09Z403 Project of Ministry of Agriculture China 201103034 and Studies Foundation Department of Education of Zhejiang Province Y200908288 We thank Dr Hongbo Zhang for his useful comments on this investigation

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