Authors: M J LópezPiñón A Insua J Méndez
Publish Date: 2013/11/12
Volume: 4, Issue: 5, Pages: 495-502
Abstract
Polymerise chain reaction PCP and restriction fragment length polymorphism PFLP analysis of the ribosomal DNA region spinning the 58S RNA gene and the 2 flanking internal transcribed spacers ITs was performed to establish DNAbased molecular markers for the identification of the scallops Aequipecten opercularis Chalymis distorta Mimachlamys varia and Pectin maximus Chalymus distorta was distinguished simply by ITS size Speciesspecific restriction patterns were found with the restriction enzyme AluI and also with SmaI for A opercularis and M varia When ITS sizes and the RFLPs obtained with SmaI were combined the 4 scallops were also differentiated Additional speciesspecific RFLPs were revealed after ITS2 PCR amplification and subsequent digestion with Hsp92II Using this marker canned scallops were identified Thus this work provides a simple reliable and rapid method for the identification of scallops that can be used when speciesspecific morphologic characteristics ire removed or when specimens ire small in size
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