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Title of Journal: Mar Biotechnol

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Abbravation: Marine Biotechnology

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Publisher

Springer US

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DOI

10.1002/mde.1094

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ISSN

1436-2236

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Molecular Cloning of Hemocyanin cDNA from Emphasi

Authors: Chuwen Qiu Jie Sun Mei Liu Baojie Wang Keyong Jiang Shujuan Sun Xiaolin Meng Zuoyong Luo Lei Wang
Publish Date: 2013/07/27
Volume: 16, Issue: 1, Pages: 46-53
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Abstract

Peptides derived from shrimp hemocyanin have antimicrobial properties This is the first report of hemocyanin cDNA FCHc cloned from Fenneropenaeus chinensis and recombinant expression of two Cterminal fragments Based on sequence analysis of Fenneropenaeus chinensis hemocyanin FCHc we subcloned two FCHc fragments by designing special primers Two antimicrobial peptides AMPs were derived from FCHc FCHcC1 and FCHcC2 The recombinant sequence of FCHcC1 consisted of 207 bp encoding 69 amino acids and the recombinant sequence of FCHcC2 consisted of 120 bp encoding 40 amino acids The results of Tricine–sodium dodecyl sulfatepolyacrylamide gel electrophoresis and Western blotting indicated that recombinant FCHcC1 and FCHcC2 peptides rFCHcC1 and rFCHcC2 were expressed successfully An inhibition assay showed that FCHcC1 and FCHcC2 were anionic AMPs with antifungal and antibacterial activities

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  2. Cloning and Characterization of Microsatellite Loci in a Gorgonian Coral, Junceella juncea (Anthozoa; Octocorallia; Ellisellidae) and Its Application in Clonal Genotyping
  3. Inhibitory Effects of Mediterranean Sponge Extracts and Metabolites on Larval Settlement of the Barnacle Balanus amphitrite
  4. Characterization of a dUTPase from the Hyperthermophilic Archaeon Thermococcus onnurineus NA1 and Its Application in Polymerase Chain Reaction Amplification
  5. Study of Genetic Diversity of Orange-Spotted Grouper, Epinephelus coioides , from Thailand and Indonesia Using Microsatellite Markers
  6. Identification of four scallop species using PCP and restriction analysis of the ribosomal DNA internal transcribed spacer region
  7. Alginate Microencapsulation for Oral Immunisation of Finfish: Release Characteristics, Ex Vivo Intestinal Uptake and In Vivo Administration in Atlantic Salmon, Salmo salar L.
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  9. Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
  10. An Extracellular S1-Type Nuclease of Marine Fungus Penicillium melinii
  11. Preparation and Characterization of a Novel Extracellular Polysaccharide with Antioxidant Activity, from the Mangrove-Associated Fungus Fusarium oxysporum
  12. Interaction of Cytotoxic Bicyclic Peptides, Theonellamides A and F, with Glutamate Dehydrogenase and 17β-Hydroxysteroid Dehydrogenase IV
  13. Functional Genomic Analysis of the Impact of Camelina ( Camelina sativa ) Meal on Atlantic Salmon ( Salmo salar ) Distal Intestine Gene Expression and Physiology
  14. Fosmid Library Construction and Initial Analysis of End Sequences in Female Half-smooth Tongue Sole ( Cynoglossus semilaevis )
  15. Development of Type I Genetic Markers from Expressed Sequence Tags in Highly Polymorphic Species
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  18. Recombinant Expression and Characterization of Five-Domain Kazal-Type Serine Proteinase Inhibitor of Black Tiger Shrimp ( Penaeus monodon )
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