Authors: Shuhui Lu Yuji Ohtsuki Yoshiki Nonami Shiro Sasaguri Jiro Fujita Masashi Uomoto Fushan Tao Makoto Kobayashi Mutsuo Furihata
Publish Date: 2006/12/21
Volume: 39, Issue: 4, Pages: 214-220
Abstract
To investigate the finestructural nature of nuclear inclusions immunopositive for surfactant protein A SPA antibody staining a detailed ultrastructural study was performed as well as immunohistochemical examination of pulmonary adenocarcinomas Surgically resected tumor specimens from 31 patients were examined by immunohistochemistry focused on reactivity to SPA and thyroid transcription factor 1 TTF1 antibodies Only cases with 5 positive nuclear inclusions in cancer cells were considered positive some of which were examined by electron microscopy Immunohistochemically 6 of 31 cases were doubly positive for SPA and TTF1 antibodies On electron microscopy SPApositive nuclei contained diffuse or globular fine granular substance as inclusions Both types of globular and diffuse inclusions were sometimes connected to the inner nuclear membrane in association with fragmented or stacked membranous structures The findings of this study suggested that nuclear inclusions positive for SPA antibody staining in adenocarcinomas of the lung were derived from accumulated content in the perinuclear cistern resembling pseudoinclusion processes and composed of proteins antigenically crossreactive with SPA
Keywords: