Plant regeneration from zygotic embryoderived emb

Authors: Sung Ran Min Jang Ryol Liu Suk Weon Kim

Publish Date: 2007/01/31

Volume: 1, Issue: 1, Pages: 57-60

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Abstract

Culture conditions for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Ranunculus kazusensis are described Zygotic embryos formed white nodular structures and paleyellow calluses at a frequency of 849 when cultured on halfstrength Schenk and Hildebrandt SH medium supplemented with 01 mg l−1 24dichlorophenoxyacetic acid 24D However the frequency of white nodular structure and offwhite callus formation decreased with an increasing concentration of 24D up to 10 mg l−1 when the frequency reached 25 Cell suspension cultures were established from zygotic embryoderived paleyellow calluses using halfstrength SH medium supplemented with 01 mg l−1 of 24D Upon plating onto halfstrength SH basal medium over 90 of cell aggregates gave rise to numerous somatic embryos and developed into plantlets Regenerated plantlets were successfully transplanted to potting soil and grown to maturity at a survival rate of over 90 in a growth chamber The plant regeneration system established in this study can be applied to mass propagation and conservation of this speciesThis work was supported by a grant from KRIBB Research Initiative Program and a grant no BDW0800622 from the Korea Science and Engineering Foundation The authors are thankful to the Plant Diversity Research Center of Korea for providing plant materials

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