Journal Title
Title of Journal: Plant Biotechnol Rep
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Abbravation: Plant Biotechnology Reports
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Authors: Kyung Il Kim Ha Young Chung Ki Hyun Yoo JongHwa Park Hyun Ho Lee Yeon Ju Soek Ki Sung Ko Hyung Sik Kang Kyung Jin Lee DooByoung Oh Young Hee Joung In Sik Chung
Publish Date: 2012/02/18
Volume: 6, Issue: 3, Pages: 233-242
Abstract
We describe expression and characterization of recombinant human colorectal cancer antigen GA7332 fused to Fc fragment of antibody GA7332Fc using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves Recombinant GA7332Fc/KDEL with a molecular mass of ~68 kDa was transiently expressed The level of expression of GA7332Fc with ER retention signal KDEL GA7332Fc/KDEL in the expression vector system was 096 of total soluble proteins Recombinant GA7332Fc/KDEL was purified using an affinity chromatography Mice immunized with recombinant GA7332Fc/KDEL mounted a strong GA7332Fc/KDELspecific serum antibody response Vaccination of plantderived recombinant GA7332Fc/KDEL regressed tumor volumes in BALB/c mice The population of activatedT and NKT cells increased notably in lymph node spleen and tumorinfiltrating lymphocytes derived from the tumorregressed mice Taken together recombinant GA7332Fc/KDEL expressed in plants can be used as an effective experimental immunogen for research in cancer vaccine developmentThis research was supported by a grant from the Basic Science Research Program through the National Research Foundation of Korea NRF funded by the Ministry of Education Science and Technology 20110003112 and a Grant 111096031HD110 from Korea Institute of Planning and Evaluation for Technology in Food Agriculture Forestry and Fisheries
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