Journal Title
Title of Journal: Plant Biotechnol Rep
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Abbravation: Plant Biotechnology Reports
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Authors: Ai Hasegawa Asami Kurita Shinji Hayashi Takeshi Fukumoto Hamako Sasamoto
Publish Date: 2012/08/17
Volume: 7, Issue: 2, Pages: 205-209
Abstract
The effects of sea salts NaCl KCl MgCl2 MgSO4 and CaCl2 on the growth of protoplast cultures of two mangrove species Sonneratia alba and Avicennia alba were investigated using 96well culture plates Plants of these two species naturally grow at the seaward side of a mangrove forest Cotyledon protoplasts of S alba showed halophilic nature to NaCl KCl and MgCl2 at low concentrations 10–50 mM when cultured in Murashige and Skoog’s MS medium containing 06 M mannitol CaCl2 at a concentration higher than 25 mM was inhibitory to cell growth On the other hand in protoplast culture of A alba suspension cells which were induced from cotyledon tissues in the modified amino acid mAA medium containing 12 M sorbitol tolerance to NaCl MgCl2 and MgSO4 were observed at a wide range of concentrations up to 400 mM CaCl2 was always inhibitory for cell divisions in A alba but stimulatory for spherical enlargement of cells However no difference in cell enlargement was observed among other salts Similarity and difference in reactivity to salts between protoplasts and suspension cells from our previous studies were discussed in relation to the site of salt tolerance or halophilic adaptation within mangrove cells For protoplast cultures the sites for response of S alba and A alba are located in the cytoplasm and/or the cell membrane
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