Journal Title
Title of Journal: Plant Biotechnol Rep
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Abbravation: Plant Biotechnology Reports
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Authors: MiJeong Ahn Seol Ah Noh SunHwa Ha Kyoungwhan Back Shin Woo Lee Jung Myung Bae
Publish Date: 2011/10/28
Volume: 6, Issue: 2, Pages: 133-140
Abstract
One of the drawbacks in inducing the synthesis of ketocarotenoids in transgenic plants is the accompanying decrease in βcarotene level especially in transgenic plants overexpressing only a βcarotene ketolase In an attempt to overcome this drawback we isolated a βcarotene ketolase HpBkt cDNA from Haematococcus pluvialis and generated transgenic carrot plants overexpressing HpBkt cDNA under the control of the ibAGP1 promoter and its transit peptide sequence Semiquantitative reverse transcriptasePCR with total RNAs extracted from taproot tissues of five lines of the generated HpBkttransgenic carrot plants revealed variable levels of the inserted HpBkt transcript ranging from very low to high All of the HpBkttransgenic carrot plants tested produced astaxanthin resulting in a color change from the lightorange coloration of wildtype taproots to a darkorange–reddish coloration in the taproots of the HpBkttransgenic plants The astaxanthin content was not correlated with the HpBkt transcript level Overexpression of HpBkt caused an increase in the transcript levels of the endogenous carotenogenic genes including phytoene synthase 1 PSY1 phytoene synthase 2 PSY2 lycopene βcyclase 1 LCYB1 and βcarotene hydroxylase 1 CHXB1 which resulted in elevated βcarotene levels in the HpBkttransgenic plants range 13 to 25fold compared to the wildtype plants These results demonstrate that HpBktoverexpressing carrot plants under the control of the ibAGP1 promoter and its transit peptide are capable of both newly synthesizing ketocarotenoids and enhancing their βcarotene levelThis work was supported by Grants No PJ008076 and No PJ008103 to JM Bae No PJ008119 to MJ Ahn No PJ008097 to SW Lee from the NextGeneration BioGreen 21 Program Rural Development Administration Republic of Korea and a research fund of the National Institute of Agricultural Science PJ0068342011 to SH Ha
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