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Title of Journal: Tree Genetics Genomes

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Abbravation: Tree Genetics & Genomes

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Springer-Verlag

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DOI

10.1007/s10833-005-4176-1

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1614-2950

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Rapid transcriptional response of apple to fire bl

Authors: John L Norelli Robert E Farrell Carole L Bassett Angela M Baldo Donna A Lalli Herb S Aldwinckle Michael E Wisniewski
Publish Date: 2008/06/27
Volume: 5, Issue: 1, Pages: 27-40
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Abstract

Fire blight caused by the bacterium Erwinia amylovora is a destructive disease of many tree and shrub species of the Rosaceae Suppression subtractive cDNA hybridization SSH was used to identify genes that are differentially up and downregulated in apple Malus x domestica in response to challenge with E amylovora cDNA libraries were constructed from E amylovora and mockchallenged apple leaf tissue at various time intervals after challenge treatment ranging from 025 to 72 h postinoculation hpi and utilized in SSH Gel electrophoresis of PCRamplified SSH cDNAs indicated a greater quantity and size diversity in the downregulated EST population at early times after challenge 1 and 2 hpi compared to early upregulated sequences and to sequences downregulated at later 24 and 48 hpi times after challenge A total of 468 nonredundant Malus ESTs isolated by SSH in response to E amylovora challenge were characterized by bioinformatic analysis Many of ESTs identified following E amylovora challenge of apple were similar to genes previously reported to respond to bacterial challenge in Arabidopsis thaliana The results indicate that there was a substantial early 1 and 2 hpi transcriptional response in apple to fire blight disease involving both the down and upregulation of host genes Additionally genes identified responding to fire blight challenge early 1 and 2 hpi differed from those identified later 25 48 and 72 hpi in the infection processWe gratefully acknowledge Wilbur Hershberger USDA ARS Kearneysville WV USA for valuable assistance with conducting biological challenge experiments isolating RNA from challenge tissues cloning PCRamplified SSHcDNAs and editing of SSHEST sequences and for bioinformatic analyses Dr David Needleman USDA ARS Wyndmoor PA USA of the Eastern Regional Research Center’s Nucleic Acid Facility for sequencing the SSHESTs Greg Richart The Pennsylvania State University York USA for assistance with SSH Jing Ma USDA ARS Kearneysville USA for assistance with semiquantitative RTPCR John McGraw USDA ARS Kearneysville USA for assistance with sequence editing and bioinformatics and Dr Zuping Yang USDAARS Kearneysville USA for assistance with cloning PCRamplified SSHcDNAs The project was supported by the National Research Initiative of the USDA Cooperative State Research Education and Extension Service grant number 20053530015462 Additional support was provided by NutriCore NE


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Other Papers In This Journal:

  1. Genetic growth parameters and morphological traits of canker-resistant cypress clones selected for timber production
  2. Characterisation and inheritance of nuclear microsatellite loci for use in population studies of the allotetraploid Salix alba – Salix fragilis complex
  3. Performance differences among ex situ native-provenance collections of Pinus radiata D. Don. 1: potential for infusion into breeding populations in Australia and New Zealand
  4. Genetic variation in basic density and modulus of elasticity of coastal Douglas-fir
  5. Natural variation, functional divergence, and local adaptation of nucleotide binding site sequences in Rhododendron (Ericaceae)
  6. Genetic structure in Pinus cembra from the Carpathian Mountains inferred from nuclear and chloroplast microsatellites confirms post-glacial range contraction and identifies introduced individuals
  7. Rosaceae conserved orthologous sequences marker polymorphism in sweet cherry germplasm and construction of a SNP-based map
  8. Development and functional annotation of an 11,303-EST collection from Eucalyptus for studies of cold tolerance
  9. Genetic variation of Central European oaks: shaped by evolutionary factors and human intervention?
  10. Estimation of genetic parameters and prediction of breeding values for apple fruit-quality traits using pedigreed plant material in Europe
  11. Identification and genetic characterization of a quantitative trait locus for adventitious rooting from apple hardwood cuttings
  12. The study of genetic diversity patterns of Coffea commersoniana , an endangered coffee species from Madagascar: a model for conservation of other littoral forest species
  13. Quantitative trait loci for foliar terpenes in a global eucalypt species
  14. Construction of a high density linkage map and its application in the identification of QTLs for soluble sugar and organic acid components in apple
  15. Molecular cloning and analysis of apple HcrVf resistance gene paralogs in a collection of related Malus species

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