Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Tree Genetics Genomes

Search In Journal Title:

Abbravation: Tree Genetics & Genomes

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1007/bf00036285

Search In DOI:

ISSN

1614-2950

Search In ISSN:
Search In Title Of Papers:

Characterisation and inheritance of nuclear micros

Authors: Robert Andrew King Sarah L Harris Angela Karp Jacqueline H A Barker
Publish Date: 2009/10/15
Volume: 6, Issue: 2, Pages: 247-258
PDF Link

Abstract

We present nine polymorphic di and trinucleotide repeat nuclear microsatellite markers selected specifically for their use in high throughput studies concerning the dioecious allotetraploid Salix alba–Salix fragilis willow complex These taxa and their hybrids are difficult to discriminate using morphological characters Thus multiplex reactions were developed for these microsatellite loci and their effectiveness to distinguish individuals especially hybrids and their inheritance patterns in controlled crosses were determined All loci displayed disomic–monogenic inheritance which allowed for the genotype data to be analysed as for a diploid organism The nine loci produced a total of 67 alleles mean 74 alleles per locus range 3–11 alleles in a reference panel of 57 individuals from two germplasm collections and natural populations Gene diversity values as measured by the expected heterozygosity ranged from 0000–0820 A total of 53 distinct multilocus genotypes were observed and ordination analysis revealed three separate clusters corresponding to S alba S fragilis and hybrids The microsatellite loci described here will be used in population genetic studies to investigate genetic variation gene flow levels of hybridisation and the extent of introgression in natural populations of the S alba–S fragilis complex They are also useful for clonal identification conservation and sustainable management of germplasm collections genetic mapping and the selection of individuals and/or certification of controlled crosses for breeding programmesThis research was funded by the European Commission Framework V Programme contract no QLK3CT200030227 The authors wish to thank Steve Hanley and Kevin Lindegaard for help with marker development and controlled crosses respectively and Karolein van Puyvelde and Ludwig Triest at Vrije Universiteit Brussel for collection and DNA extraction of material from the INBO collection Rothamsted Research receives grantaided support from the Biotechnology and Biological Sciences Research Council UK

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Genetic growth parameters and morphological traits of canker-resistant cypress clones selected for timber production
  2. Performance differences among ex situ native-provenance collections of Pinus radiata D. Don. 1: potential for infusion into breeding populations in Australia and New Zealand
  3. Genetic variation in basic density and modulus of elasticity of coastal Douglas-fir
  4. Natural variation, functional divergence, and local adaptation of nucleotide binding site sequences in Rhododendron (Ericaceae)
  5. Genetic structure in Pinus cembra from the Carpathian Mountains inferred from nuclear and chloroplast microsatellites confirms post-glacial range contraction and identifies introduced individuals
  6. Rosaceae conserved orthologous sequences marker polymorphism in sweet cherry germplasm and construction of a SNP-based map
  7. Development and functional annotation of an 11,303-EST collection from Eucalyptus for studies of cold tolerance
  8. Genetic variation of Central European oaks: shaped by evolutionary factors and human intervention?
  9. Estimation of genetic parameters and prediction of breeding values for apple fruit-quality traits using pedigreed plant material in Europe
  10. Identification and genetic characterization of a quantitative trait locus for adventitious rooting from apple hardwood cuttings
  11. The study of genetic diversity patterns of Coffea commersoniana , an endangered coffee species from Madagascar: a model for conservation of other littoral forest species
  12. Quantitative trait loci for foliar terpenes in a global eucalypt species
  13. Construction of a high density linkage map and its application in the identification of QTLs for soluble sugar and organic acid components in apple
  14. Molecular cloning and analysis of apple HcrVf resistance gene paralogs in a collection of related Malus species
  15. Rapid transcriptional response of apple to fire blight disease revealed by cDNA suppression subtractive hybridization analysis
Search Result: