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Title of Journal: J Appl Electrochem

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Abbravation: Journal of Applied Electrochemistry

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Springer Netherlands

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DOI

10.1016/0016-6480(65)90076-6

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1572-8838

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Electrochemical study of selfassembled cysteine m

Authors: Ignasi Sirés Marina Delucchi Marco Panizza Rico Ricotti Giacomo Cerisola
Publish Date: 2009/01/08
Volume: 39, Issue: 11, Pages: 2275-
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Abstract

Selfassembled monolayers of cysteine CYST have been constructed on polycrystalline gold polyAu electrode at different immersion times t immer in order to characterize them by cyclic voltammetry and electrochemical impedance spectroscopy EIS Oxidative desorption experiments allowed the calculation of the surface coverage whereas reductive desorption informed about the binding of CYST to different gold domains Desorption products showed different diffusion ability The maximum coverage was already achieved at short t immer although progressive reordering of CYST molecules led to a relevant decrease of the defects at long time as ascertained by EIS Functionalization of polyAuCYST electrodes with the enzyme microperoxidase MP11 performed by following two different binding methods showed that the enzymatically catalyzed reduction of H2O2 is enhanced when the –COOH residues of the MP11 undecapeptide chain are firstly activated and then linked to the –NH2 functional groups of the nonactivated CYST layer formed on the polyAu surface

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