Authors: G Rezaei Behbehani A Divsalar A A Saboury R Hajian Z Rezaei E Yahaghi L Barzegar
Publish Date: 2010/08/26
Volume: 39, Issue: 8, Pages: 1142-1152
Abstract
A thermodynamic study on the interaction of bovine carbonic anhydrase II CAII with cobaltII and ironIII ions was made using isothermal titration calorimetry ITC at 30015 K and 31015 K in Tris buffer solutions at pH=75 The enthalpies of interaction of Co2++ CAII and Fe3++ CAII are reported and analyzed in terms of the extended solvation theory The results indicate that there are three identical and noncooperative binding sites for Co2+ and Fe3+ ions Binding of these ions with CAII occurs exothermically with dissociation equilibrium constants of 8715 and 9100 μmol⋅L−1 at 30015 K for Co2+ and Fe3+ respectivelyThis article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team
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