Journal Title
Title of Journal: J Solution Chem
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Abbravation: Journal of Solution Chemistry
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Authors: Ashwini H Hegde B Sandhya Shankara S Kalanur J Seetharamappa
Publish Date: 2011/01/11
Volume: 40, Issue: 2, Pages: 182-197
Abstract
The mechanism of interaction of an antiallergic drug cetirizine dihydrochloride with the proteins bovine and human serum albumins has been investigated by spectrofluorimetry FTIR UVvis absorption and circular dichroism methods at physiological conditions The drug was found to quench the fluorescence intensity of the protein through a dynamic quenching mechanism Various binding parameters were evaluated based on fluorescence quenching studies carried out at 293 K 301 K and 308 K The nature of the binding force operating between the drug and protein was proposed based on the measured thermodynamic parameters A change in the secondary structure of the protein was evident from the circular dichroism measurements wherein the αhelicity decreased from 6569 to 5123 and from 427 to 3855 for the CTZ–BSA and CTZ–HSA systems respectively Based on Förster’s theory of nonradiative energy transfer the distance between the protein and drug was determined The common blood plasma ions K+ Cu2+ Ni2+ Mn2+ and Co2+ were found to influence the binding of the drug to the protein Displacement experiments were carried out to determine the binding site for drug on the protein These studies coupled with other spectroscopic results revealed that the drug was bound to the hydrophobic pocket located in sub domain IIA of site I
Keywords:
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Other Papers In This Journal:
- Ab Initio Molecular Dynamics Simulation of Infrared Absorption Spectra of H 3 O + and H 5 O 2 + in Nonaqueous Solutions of Trifluoromethanesulfonic Acid Hydrates
- Modified Screen-Printed Electrode for Potentiometric Determination of Copper(II) in Water Samples
- Inclusion Complexes of Cyclodextrins with Galangin: a Thermodynamic and Reactivity Study
- Determination of Activation Energy for the Diffusion of Fe 3+ Ions in Agar Gel Medium Containing Various Transition Metal Sulfates
- NMR Study of the Exchange Kinetics of 30-Crown-10 Complexes with Sr 2+ and Ba 2+ Cations and Crystal Structure of the 30-Crown-10 Complex with Barium Perchlorate
- A Study of the Excess Properties of Aliphatic Chlorinated Compounds with Benzylalcohol at Various Temperatures
- Liquid–Liquid Equilibria of the Methanol + Toluene + Methylcyclohexane Ternary System at 278.15, 283.15, 288.15, 293.15, 298.15 and 303.15 K
- Complexation of Am(III) and Nd(III) by 1,10-Phenanthroline-2,9-Dicarboxylic Acid
- Diffusion Coefficients for the Ternary System Water + Chloroform + Acetic Acid at 25 °C
- Electroluminescent Properties of LECs Based on Ionic Transition Metal Complexes Using Tetrazole-Based Ancillary Ligand
- High-Pressure Densities and Derived Volumetric Properties (Excess, Apparent and Partial Molar Volumes) of Binary Mixtures of Methanol + [BMIM][PF 6 ]
- Solvent Effects on the Protonation Constants of Some α -Amino Acid Esters in 1,4-Dioxane–Water Mixtures
- Lanthanide Complexes with Allyl Acetoacetate in Mixed Water–Organic Media: Formation, Stability and Bonding
- A Study on Host–Guest Complexation of 5-Amino-2-Mercaptobenzimidazole with β -Cyclodextrin
- The Mixing Enthalpy Interaction Coefficients of N , N ′-Hexamethylenebisacetamide with l -Alanine and l -Serine in Aqueous Glucose Solutions at 298.15 K
- Effect of Temperature on Salt–Salt Aqueous Biphasic Systems: Manifestations of Upper Critical Solution Temperature
- Studies on Intermolecular Interactions in Liquid Mixtures of Alkoxyalkanols and Branched Alcohols by Volumetric and Acoustic Measurements at Different Temperatures
- Li + Transport Mechanism in Oligo(Ethylene Oxide)s Compared to Carbonates
- Thermodynamics of concentrated electrolyte mixtures. I. Activity coefficients in aqueous NaCl−CaCl 2 at 25°C
- Study of the Association Behavior Between Bromophenol Blue and Octylphenol Polyoxyethylene Ether (10) in Aqueous Solution and the Solubilization of Bromophenol Blue by Micelles
- Kinetics and Mechanism of the Reactions Between Triphenylphosphine, Dialkyl Acetylenedicarboxilates and a NH-Acid, Pyrazole, by UV Spectrophotometry
- Isopiestic Determination of the Osmotic and Activity Coefficients of Li 2 SO 4 (aq) at T =298.15 and 323.15 K, and Representation with an Extended Ion-Interaction (Pitzer) Model
- Volumetric and Transport Properties of Ternary Mixtures Containing 1-Butanol or 1-Pentanol, Triethylamine and Cyclohexane at 303.15 K: Experimental Data, Correlation and Prediction by the ERAS Model
- First Hydrolysis Constant of Lutetium (III) by Solvent Extraction
- Volumetric Properties of the Nucleoside Thymidine in Aqueous Solution at T = 298.15 K and p = (10 to 100) MPa
- Spinodal Composition of the System Water + Chloroform + Acetic Acid at 25 °C
- Complexation Studies of Pyridyl Sulfonamide Ligands for Sensing Zinc and Copper Ions
- A Thermodynamic Study on the Binding of Cobalt(II) and Iron(III) Ions with Bovine Carbonic Anhydrase II at Different Temperatures
- Solubility of Omeprazole Sulfide in Different Solvents at the Range of 280.35–319.65 K
- Chemical Equilibria Modeling of Calcium Phosphate Precipitation and Transformation in Simulated Physiological Solutions
- Isopiestic Determination of the Osmotic and Activity Coefficients of the { y Na 2 HPO 4 + (1 − y )K 2 HPO 4 }(aq) System at T = 298.15 K
- Ionic Strength Effect on the Stability of the V(V) + IDA Complex
- Viscosities for Ionic Liquid Binary Mixtures with a Common Ion
- Kinetics and Mechanism for Hydrolysis of α -Amino Acid Esters in Mixed Ligand Complexes with Zn(II)–Nitrilo-tris(methyl phosphonic Acid)
- The Oxidation of Iron(II) with Oxygen in NaCl Brines
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