Combination Efficacy of Voriconazole and Amphoteri

Authors: Eriques Gonçalves Silva Claudete Rodrigues Paula Amanda Latercia Tranches Dias Marilene Rodrigues Chang Luciana da Silva Ruiz Valderez Gambale Renato Araujo Prates Martha Simões Ribeiro

Publish Date: 2010/10/24

Volume: 171, Issue: 4, Pages: 261-266

PDF Link

Abstract

The therapeutic efficacy of amphotericin B and voriconazole alone and in combination with one another were evaluated in immunodeficient mice BALB/cSCID infected with a fluconazoleresistant strain of Cryptococcus neoformans var grubii The animals were infected intravenously with 3 × 105 cells and intraperitoneally treated with amphotericin B 15 mg/kg/day in combination with voriconazole 40 mg/kg/days Treatment began 1 day after inoculation and continued for 7 and 15 days postinoculation The treatments were evaluated by survival curves and yeast quantification CFUs in brain and lung tissues Treatments for 15 days significantly promoted the survival of the animals compared to the control groups Our results indicated that amphotericin B was effective in assuring longestterm survival of infected animals but these animals still harbored the highest CFU of C neoformans in lungs and brain at the end of the experiment Voriconazole was not as effective alone but in combination with amphotericin B it prolonged survival for the secondlongest time period and provided the lowest colonization of target organs by the fungus None of the treatments were effective in complete eradication of the fungus in mice lungs and brain at the end of the experiment

Keywords: