In Vitro Evaluation of Phospholipase Proteinase

Authors: Yi Ping Ge Gui Xia Lu Yong Nian Shen Wei Da Liu

Publish Date: 2011/06/23

Volume: 172, Issue: 6, Pages: 429-438

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Abstract

The aim of this study is to characterize extracellular phospholipase proteinase and esterase activities of Candida parapsilosis and C metapsilosis isolated from clinical sources Using PCRrestriction fragment length polymorphism PCR–RFLP of the secondary alcohol dehydrogenase SADH gene fragment we identified 20 as C parapsilosis and 11 as C metapsilosis from 31 isolates of C parapsilosis species complex No C orthopsilosis was identified A significantly high isolation frequency of C metapsilosis 355 was observed Subsequent evaluation of enzymatic profile showed that 905 of C parapsilosis and 917 of C metapsilosis isolates were phospholipase producers No difference in phospholipase activity was observed between two species In terms of proteinase 810 of C parapsilosis and 833 of C metapsilosis isolates were positive A higher level of proteinase activity was detected in C parapsilosis A remarkably high proportion of both C parapsilosis and C metapsilosis isolates exhibited strong phospholipase and proteinase activities suggesting that the production of these two enzymes might be common for them On the other hand both species similarly displayed rare esterase activity with only one C parapsilosis and two C metapsilosis isolates being positive Our data may further add to the confusion concerning the hydrolytic enzymatic activities of the C parapsilosis complex and a wider collection of isolates and standardized methods may help to address the issue

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