Authors: Ahn Na Koo Il Keun Kwon Sang Cheon Lee SoonKi Lee HyeongSeob Kim YiHyung Woo SeongHyun Jeon JiHwa Chae KeWon Kang
Publish Date: 2010/11/26
Volume: 18, Issue: 10, Pages: 1030-1036
Abstract
Novel porous polyLlactide PLLA scaffolds with surfaceimmobilized nanohydroxyapatite NHAp were synthesized for effective bone regeneration NHapbearing surface phosphate functionalities were immobilized chemically on the pore surface of aminetreated PLLA scaffolds Thermogravimetric analysis TGA showed that 24 wt of NHAp had been immobilized on the scaffold pore surface Fieldemission scanning electron microscopy FESEM and Xray photoelectron spectroscopy XPS confirmed that NHAp was exposed markedly on the pore surfaces of scaffolds compared to the conventional scaffold fabricated by a bulkmixing process of PLLA and NHAp The PLLA scaffold with surfaceimmobilized NHAp had a higher surface Ca atomic ratio 46 than that on the pore surface of the bulkmixed PLLA/NHAp scaffold Ca = 04 The porous PLLA scaffolds with surfaceimmobilized NHAp provided favorable environments for enhanced in vivo bone tissue growth which was estimated by hematoxylin and eosin HE staining for organic bone tissues and alizarin red S staining for inorganic bone minerals These results suggest that the NHAp immobilization approach may produce many useful scaffolds with pore surfaces featuring excellent bone tissueregenerative properties
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