Authors: ByungChul Kim Jae Hyung Kim Hyo Jung An Wook Byun JaeHong Park Il Keun Kwon Jin Seok Kim YuShik Hwang
Publish Date: 2014/02/12
Volume: 22, Issue: 3, Pages: 287-296
Abstract
In cartilage tissue engineering various technical approaches using postnatal stem cells threedimensional 3D scaffolds composed of synthetic or natural polymers and culture systems have been applied to develop 3D cartilagelike tissue In this study scaffoldfree 3D microcartilagelike tissue was developed via microwellmediated cell spheroid formation and 3D dynamic chondrogenic culture in a bioreactor First homogenous microcell spheroids were generated by the selfcondensation of adipose tissuederived stem cells ADSCs in microfabricated polyethylene glycol PEG hydrogel microwells Next chondrogenic differentiation of the microADSC spheroids was induced in the presence of transforming growth factorbeta 3 under dynamic 3D culture conditions using a high aspect ratio vessel bioreactor Several hundred viable ADSC spheroids could be generated at a time from ADSC culture in PEG microwells The 3D dynamic chondrogenic culture of ADSCs in the bioreactor facilitated the chondrogenic mRNA expression of proteins such as sox9 runx2 osterix type II collagen and aggrecan and the well deposition of glycosaminoglycan and type II collagen which finally generated microcartilagelike tissue Therefore the hydrogel microwell arrays could be useful for efficiently deriving initial cell condensationmediated chondrogenic differentiation and for developing 3D cellbased microcartilagelike tissue with stem cells in a controlled manner
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