Isolation and characterization of seventynine ful

Authors: MingBing Zhou Hao Zhong DingQin Tang

Publish Date: 2010/12/17

Volume: 124, Issue: 5, Pages: 607-617

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Abstract

Marinerlike elements MLEs are the most diverse and widespread transposable elements with members of the MLE superfamily found in fungi plants ciliates and animals In a previous study we characterized 82 MLE transposase gene fragments average length 383 bp in 44 bamboo species indicating that MLEs are widespread abundant and diverse in the Bambusoideae subfamily In this study we isolated 79 fulllength MLE transposase genes from 63 bamboo species representing 38 genera in six subtribes mainly found in China The transposases were highly conserved mostly uniform in length and contained intact DNAbinding motifs and DD39D catalytic domains with few notable frameshift indel and nonsense mutations This suggested the MLEs are probably still mobile not yet affected by vertical inactivation A phylogenetic tree of the Bambusoideae subfamily established using ribosomal DNA internal transcribed spacer sequences was incongruent with a second tree based on the MLE transposase genes This evidence together with the presence of nearidentical MLEs in distantly related species and diverse MLEs in closely related species indicates that MLEs have evolved in a distinct manner probably independently of speciation events in the subfamily The evolution and diversity of MLE transposase genes in the Bambusoideae subfamily is discussedWe extend our thanks to Ma Naixun from the Research Institute of Subtropical Forestry and Xingchun Lin from the Key Lab for Modern Silvicultural Technology of Zhejiang Province for their valuable advice on sampling strategies We are also indebted to Yoshinaga Katsuhiko from the World Bamboos Research Center Fukuoka Japan Changpin Zhou from the Anji Bamboo Museum Garden Mingshui Zhao from the Tianmu Mountain World Biosphere Reserve and Yaoguo Zhou from Huaan Bamboo Garden for help in the collection of the bamboo materials used in this investigation This work was supported by a special grant from the National Natural Science Foundation of China grant no 30371181 and 30771753 and Talents Program of Natural Science Foundation of Zhejiang Province grant no Y3080002 and R303420

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