Journal Title
Title of Journal: J Plant Res
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Abbravation: Journal of Plant Research
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Authors: Ai Hasegawa Tomoya Oyanagi Reiko Minagawa Yoshiharu Fujii Hamako Sasamoto
Publish Date: 2014/07/26
Volume: 127, Issue: 6, Pages: 755-761
Abstract
A bioassay method for allelopathy the ‘protoplast coculture method’ was developed to study the relationship between salt tolerance and allelopathy of three mangrove species Sonneratia alba S caseolaris and S ovata Plants of S alba grow in the seawardside high salinity region and plants of the latter two species grow in upstreamside regions of a mangrove forest respectively Effects of five sea salts NaCl KCl MgCl2 MgSO4 and CaCl2 on the growth of the suspension cells of the latter two species were first investigated by a smallscale method using 24well culture plates S ovata cells showed higher tolerance than S caseolaris cells to NaCl and other salts but were not as halophilic as S alba cells Protoplasts isolated from suspension cells were cocultured with lettuce protoplasts in Murashige and Skoog’s MS basal medium containing 1 μM 24dichlorophenoxyacetic acid 01 μM benzyladenine 3 sucrose and 06–08 M osmoticum S caseolaris protoplasts had a higher inhibitory effect on lettuce protoplast cell divisions than S alba protoplasts at any lettuce protoplast density and the effect of S ovata was intermediate between the two These results were similar to those obtained from a different in vitro bioassay method for allelopathy the ‘sandwich method’ with dried leaves The inverse relationship between allelopathic activity and salt tolerance in suspension cells of Sonneratia mangroves is discussed
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