Journal Title
Title of Journal: J Plant Res
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Abbravation: Journal of Plant Research
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Authors: Hikaru Sawano Takuma Matsuzaki Tomoyuki Usui Midori Tabara Akihito Fukudome Akihiro Kanaya Daichi Tanoue Akihiro Hiraguri Gorou Horiguchi Misato Ohtani Taku Demura Toshinori Kozaki Kazuo Ishii Hiromitsu Moriyama Toshiyuki Fukuhara
Publish Date: 2016/12/19
Volume: 130, Issue: 1, Pages: 45-55
Abstract
The model plant Arabidopsis thaliana has five doublestranded RNAbinding proteins DRB1DRB5 two of which DRB1 and DRB4 are well characterized In contrast the functions of DRB2 DRB3 and DRB5 have yet to be elucidated In this study we tried to uncover their functions using drb mutants and DRBoverexpressed lines In overexpressed lines of all five DRB genes the overexpression of DRB2 or DRB3 DRB2ox or DRB3ox conferred a downwardcurled leaf phenotype but the expression profiles of ten small RNAs were similar to that of the wildtype WT plant Phenotypes were examined in response to abiotic stresses Both DRB2ox and DRB3ox plants exhibited salttolerance When these plants were exposed to cold stress drb2 and drb3 overaccumulated anthocyanin but DRB2ox and DRB3ox did not Therefore the overexpression of DRB2 or DRB3 had pleiotropic effects on host plants Microarray and deepsequencing analyses indicated that several genes encoding key enzymes for anthocyanin biosynthesis including chalcone synthase CHS dihydroflavonol reductase DFR and anthocyanidin synthase ANS were downregulated in DRB3ox plants When DRB3ox was crossed with the pap1D line which is an activationtagged transgenic line that overexpresses the key transcription factor PAP1 Production of anthocyanin pigmentation1 for anthocyanin biosynthesis overexpression of DRB3 suppressed the expression of PAP1 CHS DFR and ANS genes DRB3 negatively regulates anthocyanin biosynthesis by modulating the level of PAP1 transcript Since two different small RNAs regulate PAP1 gene expression a possible function of DRB3 for small RNA biogenesis is discussedWe are grateful to Drs Kazuki Saito and Keiko YonekuraSakakibara at The RIKEN Center for Sustainable Resource Science for fruitful discussion The authors also thank Dr Richard A Dixon and the Arabidopsis Biological Resource Center for kindly providing Arabidopsis seeds This work was supported by the Ministry of Education Culture Sports Science and Technology MEXT of Japan a GrantinAid for Scientific Research C No 24570044 and Scientific Research on Innovative Area No 16H06435 to TF
Keywords:
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Other Papers In This Journal:
- Speciation process of Salvia isensis (Lamiaceae), a species endemic to serpentine areas in the Ise-Tokai district, Japan, from the viewpoint of the contradictory phylogenetic trees generated from chloroplast and nuclear DNA
- Extensive investigation of the sap flow of maize plants in an oasis farmland in the middle reach of the Heihe River, Northwest China
- miR156 modulates rhizosphere acidification in response to phosphate limitation in Arabidopsis
- Leaf-shape variation of Paederia foetida in Japan: reexamination of the small, narrow leaf form from Miyajima Island
- Transcriptional repression caused by Dof5.8 is involved in proper vein network formation in Arabidopsis thaliana leaves
- Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina
- Seasonal shift in factors controlling net ecosystem production in a high Arctic terrestrial ecosystem
- Methods of estimating seed banks with reference to long-term seed burial
- A study on calcium oxalate crystals in Tinantia anomala (Commelinaceae) with special reference to ultrastructural changes during anther development
- Isolation and characterization of seventy-nine full-length mariner -like transposase genes in the Bambusoideae subfamily
- The anatomy of the chi-chi of Ginkgo biloba suggests a mode of elongation growth that is an alternative to growth driven by an apical meristem
- Temporal variation in leaf nitrogen partitioning of a broad-leaved evergreen tree, Quercus myrsinaefolia
- Exosomes versus microexosomes: Shared components but distinct functions
- Signaling mechanisms of plant cryptochromes in Arabidopsis thaliana
- Endophytic colonization of barley ( Hordeum vulgare ) roots by the nematophagous fungus Pochonia chlamydosporia reveals plant growth promotion and a general defense and stress transcriptomic response
- 2013 Awards for Journal of Plant Research publications
- Functional analyses of the plant-specific C-terminal region of VPS9a: the activating factor for RAB5 in Arabidopsis thaliana
- Embryology of Pera (Peraceae, Malpighiales): systematics and evolutionary implications
- An inverse relationship between allelopathic activity and salt tolerance in suspension cultures of three mangrove species, Sonneratia alba , S. caseolaris and S. ovata : development of a bioassay method for allelopathy, the protoplast co-culture method
- Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants
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