Authors: Lara Macchioni Magdalena Davidescu Miriam Sciaccaluga Cristina Marchetti Graziella Migliorati Stefano Coaccioli Rita Roberti Lanfranco Corazzi Emilia Castigli
Publish Date: 2011/07/21
Volume: 43, Issue: 5, Pages: 507-
Abstract
Most cancer cells including GL15 glioblastoma cells rely on glycolysis for energy supply The effect of antiglycolytic bromopyruvate on respiratory parameters and viability of GL15 cells was investigated Bromopyruvate caused Δψm and MTT collapse ATP decrease and cell viability loss without involving apoptotic or necrotic pathways The autophagy marker LC3II was increased Δψm decrease was accompanied by reactive oxygen species ROS increase and cytochrome c cyt c disappearance suggesting a link between free radical generation and intramitochondrial cyt c degradation Indeed the free radical inducer menadione caused a decrease in cyt c that was reversed by Nacetylcysteine Cyt c is tightly bound to the inner mitochondrial membrane in GL15 cells which may confer protein peroxidase activity resulting in autooxidation and protein targeting to degradation in the presence of ROS This process is directed towards impairment of the apoptotic cyt c cascade although cells are committed to die
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