Fluoxetine induces lean phenotype in rat by increa

Authors: A I da Silva G R F Braz A A Pedroza L Nascimento C M Freitas D J S Ferreira R Manhães de Castro C J Lagranha

Publish Date: 2015/07/01

Volume: 47, Issue: 4, Pages: 309-318

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Abstract

The serotonergic system plays a crucial role in the energy balance regulation Energy balance is mediated by food intake and caloric expenditure Thus the present study investigated the mechanisms that might be associated with fluoxetine treatmentinduced weight reduction Wistar male rat pups received daily injections with subcutaneous fluoxetine Fxgroup or vehicle solution Ctgroup from day 1 until 21 days of age Several analyses were conducted to verify the involvement of mitochondria in weight reduction We found that body weight in the Fxgroup was lower compared to control In association to lower fat mass in the Fxgroup 25  Neither neonatal caloric intake nor food intake reveals significant differences Evaluating caloric expenditure locomotor activity and temperature after stimulus we did not observe differences in locomotor activity However we observed that the Fx group had a higher capacity to maintain body temperature in a cold environment compared with the Ctgroup Since brown adipose tissueBAT is specialized for heat production and the rate of heat production is related to mitochondrial function we found that Fxtreatment increases respiration by 36  although after addition of GDP respiration returned to Ctlevels Examining ROS production we observe that Fxgroup produced less ROS than control group Evaluating uncoupling protein UCP expression we found that Fxtreatment increases the expression by 23  Taken together our results suggest that modulation of serotonin system results in positive modulation of UCP and mitochondrial bioenergetics in brown fat tissueThe authors are thankful to Dr Elizabeth Murphy for her assistance in the discussion of this paper The authors are also grateful to Foundation for Science and Technology of the State of Pernambuco FACEPEAPQ1026409/12 for the financial support and for the scholarship to GRFB LCPN CNPq for the scholarship to AAP and CAPES for the scholarship to AIS

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