Pulmonary Inflammation and Cell Death in Mice Afte

Authors: F Astort M Sittner S A Ferraro N S Orona G A Maglione A De la Hoz D R Tasat

Publish Date: 2013/12/11

Volume: 67, Issue: 1, Pages: 87-96

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Abstract

Epidemiological studies have shown that air particulate matter PM can increase respiratory morbidity and mortality being the lungs the main target organ to PM body entrance Even more several in vivo and in vitro studies have shown that air PM has a wide toxicity spectra depending among other parameters on its size morphology and chemical composition The Reconquista River is the second most polluted river from Buenos Aires and people living around its basin are constantly exposed to its contaminated water soil and air However the air PM from the Reconquista River RRPMa has not been characterized and its biological impact on lung has yet not been assessed Therefore the present investigation was undertaken to study 1 RRPMa morphochemical characteristic and 2 RRPMa lung acute effects after intranasal instillation exposure through the analysis of three end points oxidative stress inflammation and apoptosis A single acute exposure of RRPMa 1 mg/kg body weight after 24 h caused significant p  005 enrichment in bronchoalveolar total cell number and polymorphonuclear PNM fraction superoxide anion generation production of proinflammatory cytokines TNFα and IL6 and induction of apoptosis It was also observed that in lung homogenates none of the antioxidant enzymes assayed showed differences between exposed RRPMa and control mice These data demonstrate that air PM from the Reconquista River induce lung oxidative stress inflammation and cell death therefore represents a potential hazard to human healthWe especially thank Gisela Maxia for technical assistance with SEM and EDX and Paulo H Saldiva for kindly providing the collector sampler This study was supported by the Agencia de Promoción Científica y Técnica from Argentina Grant No PICT20101660

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